26.11.2012 | Marcela Tlustá, Ján Krahulec, Stanislav Pepeliaev, Lukáš Franke, Zbyněk Černý, Jana Jílková, Molecular Biotechnology, 2012

This study addresses the influence of upstream region sequence on the strength of has operon promoter in highly encapsulated S. equi subsp. zooepidemicus (SEZ). For this purpose, seven different strains were constructed. Each strain carries a point mutation in one of the following positions ...

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26.11.2012 | J. Pedro Menvielle, Najete Safini, Sergio G. Tisminetzky, Natasa Skoko, Molecular Biotechnology, 2012

Dextran sulfate 5,000 Da (DS), a sulfated polysaccharide, has been used in recombinant mammalian cell cultures to prevent cell aggregation, thereby increasing cell viability. Previous studies using Chinese hamster ovary (CHO) suspension cultures had shown that low concentrations of DS are ...

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26.11.2012 | Yanjie Xie, Diwen Lai, Yu Mao, Wei Zhang, Wenbiao Shen, Rongzhan Guan, Molecular Biotechnology, 2012

l-Cysteine desulfhydrase (DES; EC 4.4.1.1) is the most important enzyme that catalyzes the decomposition of l-cysteine to pyruvate, ammonia, and hydrogen sulfide (H2S), the latter of which has recently been recognized as the third gasotransmitter for multiple signaling events in plants. ...

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26.11.2012 | Anuradha B. Singh, Krishna J. Mukherjee, Molecular Biotechnology, 2012

Over-expression of recombinant proteins in Escherichia coli triggers a metabolic stress response which causes a sharp decline in both growth and product formation rates post induction. We identified a key down-regulated substrate utilization gene, glycerol kinase (glpK), whose up-regulation ...

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02.11.2012 | M. Arif, F. M. Ochoa-Corona, Molecular Biotechnology, 2012

Efficient PCR amplifications require precisely designed and optimized oligonucleotide primers, components, and cycling conditions. Despite recent software development and reaction improvement, primer design can still be enhanced. The aims of this research are to understand (1) the effect on ...

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02.11.2012 | Federica Martellini, Franco Faoro, Lara Carresi, Barbara Pantera, Ivan Baccelli, Dario Maffi, Bruno Tiribilli, Franc ..., Molecular Biotechnology, 2012

Based on sequence homology, several fungal Cys-rich secreted proteins have been grouped in the cerato-platanin (CP) family, which comprises at least 40 proteins involved mainly in eliciting defense-related responses. The core member of this family is cerato-platanin, a moderately hydrophobic ...

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31.10.2012 | Alejandra Muñoz Bodnar, Adriana Bernal, Boris Szurek, Camilo E. López, Molecular Biotechnology, 2012

Pathogenic bacteria of the Xanthomonas and Ralstonia genus have developed resourceful strategies creating a favorable environment to multiply and colonize their host plants. One of these strategies involves the secretion and translocation of several families of effector proteins into the host ...

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28.10.2012 | Christoph Metzner, Feliks Kochan, John A. Dangerfield, Molecular Biotechnology, 2012

In this study, we describe a versatile, flexible, and quick method to label different families of enveloped viruses with glycosylphosphatidylinositol-modified green fluorescent protein, termed fluorescence molecular painting (FMP). As an example for a potential application, we investigated ...

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25.10.2012 | Dmitrij Hristodorov, Rainer Fischer, Lars Linden, Molecular Biotechnology, 2012

Antibodies and antibody-based drugs are currently the fastest-growing class of therapeutics. Over the last three decades, more than 30 therapeutic monoclonal antibodies and derivatives thereof have been approved for and successfully applied in diverse indication areas including cancer, organ ...

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23.10.2012 | Yunzhi Pan, Li Xiao, Alice S. S. Li, Xu Zhang, Pierre Sirois, Jia Zhang, Kai Li, Molecular Biotechnology, 2012

The development of engineered nucleases is the fruit of a new technological approach developed in the last two decades which has led to significant benefits on genome engineering, particularly on gene therapy. These applications enable efficient and specific genetic modifications via the ...

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