09.10.2012 | D. V. Novikov, T. V. Belova, E. S. Plekhanova, O. S. Yanchenko, V. V. Novikov, Molecular Biology, 2012

Recent studies have suggested that mRNA transcribed from cancer/testis genes might be used as biomarkers of cancer cells that migrate through the blood system. Using RT-PCR, we evaluated the expression of several cancer/testis mRNAs (MAGEA1-6, GAGE1-8, NY-ESO-1, SSX1, 2, and 4, XAGE1 and ...

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09.10.2012 | N. I. Gutkina, V. V. Bogachev, S. P. Kovalenko, Molecular Biology, 2012

The technique for detecting frameshift and nonsense mutations in the human BRCA1 gene has been suggested. The technique presumes the construction of recombinant plasmids where the tested DNA fragment is placed in frame with alkaline phosphatase gene of Escherichia coli (phoA). A special ...

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09.10.2012 | Y. -H. Noh, H. -S. Cho, D. -H. Kim, O. H. Kim, J. Park, S. -A. Lee, H. -S. Yang, D. -S. Sohn, W. Kim, D. Kim, Y. H. ..., Molecular Biology, 2012

We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were ...

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09.10.2012 | A. D. Perenkov, D. V. Novikov, N. A. Sakharnov, A. V. Alyasova, O. V. Utkin, A. Yu. Baryshnikov, V. V. Novikov, Molecular Biology, 2012

The CD38 gene encodes a membrane-bound protein that takes part in cell adhesion and catalyzes the formation of cyclic ADP-ribose. The presence of the full-size and alternative forms of CD38 mRNA in tumor tissue specimens from patients with colorectal cancer and tumor cell lines was analyzed ...

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09.10.2012 | D. O. Fesenko, O. V. Kalennik, V. E. Barsky, A. S. Zasedatelev, T. V. Nasedkina, Molecular Biology, 2012

A biochip has been developd that enables one to determine Y-chromosome haplogroups C, DE, G, H, I, J, L, N, O, and R in the DNA sample. SNPs M130, M145, P257, M69, U179, M304, M185, M231, M175, P224 were selected as haplogroup markers, correspondingly. The genotyping included two-round PCR ...

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09.10.2012 | Y. -Z. Cheng, T. -J. Xu, X. -X. Jin, D. Tang, T. Wei, Y. -Y. Sun, F. -Q. Meng, G. Shi, R. -X. Wang, Molecular Biology, 2012

Through multiple alignment analysis of mitochondrial tRNA-Thr and tRNA-Phe sequences from 161 fishes, new universal primers specially targeting the entire mitochondrial control region were designed. This new primer set successfully amplified the expected PCR products from various kinds of ...

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09.10.2012 | B. N. Gol’dshtein, A. M. Aksirov, D. T. Zakrzhevskaya, Molecular Biology, 2012

F1-ATPase is a catalytic part of the F1Fo-ATP synthase molecular motor. The cooperative hydrolysis of ATP at three catalytic sites of F1-ATPase is accompanied by the rotation of the central γ-subunit inside a cylinder formed by three α-subunits and three β-subunits. Experimental works of ...

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09.10.2012 | O. A. Zverkov, A. V. Seliverstov, V. A. Lyubetsky, Molecular Biology, 2012

Protein clustering is useful for refining protein annotations and searching for proteins by their phylogenetic profile. We have performed the clustering of proteins encoded in the plastoms of Rhodophyta, as well as other plastid-containing species related to the Rhodophyta branch. The ...

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09.10.2012 | O. S. Khasanova, D. A. Vagin, F. K. Khasanov, Molecular Biology, 2012

Repair of double-strand breaks (DSBs), which arise in DNA spontaneously or under the influence of external factors, is critical for cell survival. The evolutionarily conserved mechanism of error-free recombinational repair plays a major role in maintaining the genome integrity and has a ...

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09.10.2012 | D. S. Khodyrev, I. V. Pronina, S. V. Rykov, E. V. Beresneva, M. V. Freedman, T. P. Kazubskaya, V. I. Loginov, E. A. ..., Molecular Biology, 2012

To date, there are more than 2000 known human miRNAs, each of which may be involved in the regulation of hundreds of protein-coding target genes. In turn, the methylation of CpG islands affects the miRNA gene expression. Our aim was to evaluate the role of methylation in the regulation of ...

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