27.11.2012 | Yuki Matano, Tomohisa Hasunuma, Akihiko Kondo, Applied Microbiology and Biotechnology, 2012

Enzymatic hydrolysis of cellulosic material is an essential step in the bioethanol production process. However, complete cellulose hydrolysis by cellulase is difficult due to the irreversible adsorption of cellulase onto cellulose. Thus, part of the cellulose remains in crystalline form after ...

mehr

27.11.2012 | Raushan Kumar Singh, Manish Kumar Tiwari, Dongwook Kim, Yun Chan Kang, Priyadharshini Ramachandran, Jung-Kul Lee, Applied Microbiology and Biotechnology, 2012

An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg ...

mehr

27.11.2012 | Eric A. Johnson, Applied Microbiology and Biotechnology, 2012

Saccharomyces cerevisiae and several other yeast species are among the most important groups of biotechnological organisms. S. cerevisiae and closely related ascomycetous yeasts are the major producer of biotechnology products worldwide, exceeding other groups of industrial microorganisms in ...

mehr

26.11.2012 | Hiroaki Mon, JaeMan Lee, Mai Fukushima, Yudai Nagata, Mie Fujii, Jian Xu, Oumi Nishi, Kazuhiro Iiyama, Takahiro Kusakabe, Applied Microbiology and Biotechnology, 2012

Mutation and polymorphism detection by nucleases has become a more important tool in clinical and biological researches. There are several kinds of single-stranded nucleases for detecting mismatched DNAs. One of them, CEL II, was isolated from Apium graveolens and cleaves DNA with high ...

mehr

26.11.2012 | Hui Liu, Zhiming Zheng, Peng Wang, Guohong Gong, Li Wang, Genhai Zhao, Applied Microbiology and Biotechnology, 2012

Chitin synthases catalyze the formation of β-(1,4)-glycosidic bonds between N-acetylglucosamine residues to form the unbranched polysaccharide chitin, which is the major component of cell walls in most filamentous fungi. Several studies have shown that chitin synthases are structurally and ...

mehr

26.11.2012 | Jerzy Falandysz, Jan Borovička, Applied Microbiology and Biotechnology, 2012

This article reviews and updates data on macro and trace elements and radionuclides in edible wild-grown and cultivated mushrooms. A huge biodiversity of mushrooms and spread of certain species over different continents makes the study on their multi-element constituents highly challenging. A ...

mehr

26.11.2012 | Tobias Heck, Greta Faccio, Michael Richter, Linda Thöny-Meyer, Applied Microbiology and Biotechnology, 2012

The process of protein crosslinking comprises the chemical, enzymatic, or chemoenzymatic formation of new covalent bonds between polypeptides. This allows (1) the site-directed coupling of proteins with distinct properties and (2) the de novo assembly of polymeric protein networks. ...

mehr

26.11.2012 | Keith W. Y. Kwong, K. L. Ng, C. C. Lam, Yule Y. Wang, W. K. R. Wong, Applied Microbiology and Biotechnology, 2012

Bacillus subtilis is generally accepted as an inborn host candidate employed for secretory production of heterologous proteins. However, this ideal host system has never been employed for commercial production of medically useful proteins. In this communication, we report for the first time ...

mehr

26.11.2012 | Keiro Watanabe, Hiroshi Teramoto, Nobuaki Suzuki, Masayuki Inui, Hideaki Yukawa, Applied Microbiology and Biotechnology, 2012

The non-essential Corynebacterium glutamicum sigma factor, sigB, modulates global gene expression during the transition from exponential growth to the stationary phase. Utilizing a signal peptide derived from C. glutamicum R CgR_0949, a sigB disruption mutant able to secrete 3- to 5-fold more ...

mehr

26.11.2012 | Javier A. Gimpel, Stephen P. Mayfield, Applied Microbiology and Biotechnology, 2012

The basic photosynthetic apparatus is highly conserved across all photosynthetic organisms, and this conservation can be seen in both protein composition and amino acid sequence. Conservation of regulatory elements also seems possible in chloroplast genes, as many mRNA untranslated regions ...

mehr