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Due to its potential in preventing or slowing the occurrence of many diseases, resveratrol (3,5,4′-trihydroxystilbene) has attracted great research interest. The objective of this study was to identify microorganisms from selected plants that produce resveratrol and to optimize the conditions for resveratrol production. Endophytes from Merlot wine grapes (Vitis vinifera L. cv. Merlot), wild Vitis (Vitis quinquangularis Rehd.), and Japanese knotweed (Polygonum cuspidatum Siebold & Zucc.) were isolated, and their abilities to produce resveratrol were evaluated. A total of 65 isolates were obtained and 21 produced resveratrol (6–123 μg/L) in liquid culture. The resveratrol-producing isolates belonged to seven genera, Botryosphaeria, Penicillium, Cephalosporium, Aspergillus, Geotrichum, Mucor, and Alternaria. The resveratrol-producing capability decreased or was completely lost in most isolates after three rounds of subculture. It was found that only the strain Alternaria sp. MG1 (isolated from cob of Merlot using GA1 medium) had stable and high resveratrol-producing capability in all subcultures. During liquid cultivation of Alternaria sp. MG1 in potato dextrose medium, the synthesis of resveratrol began on the first day, increased to peak levels on day 7, and then decreased sharply thereafter. Cell growth increased during cultivation and reached a stable and high level of biomass after 5 days. The best fermentation conditions for resveratrol production in liquid cultures of Alternaria sp. MG1 were an inoculum size of 6 %, a medium volume of 125 mL in a 250-mL flask, a rotation speed of 101 rpm, and a temperature of 27 °C.
Endothelial nitric oxide synthase polymorphism G298T in association with oxidative DNA damage in coronary atherosclerosis
Content Type Journal Article
Category Research Note
Pages 1-4
DOI 10.1007/s12041-012-0183-1
Authors
RAJESH G. KUMAR, Department of Genetics, Osmania Univers ... mehr
A micropropagation protocol was developed for multiplication of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) using nodal explants. The maximum shoot regeneration was observed on low level of BAP (0.1 mg l−1) or kinetin (0.5 mg l−1). BA was recorded to be better than kinetin in terms of ... mehr
The objective of this study was to evaluate the activity, kinetic behavior, and thermal inactivation kinetics of peroxidase (POD) in aqueous extracts from two kinds of milk ripe stage corn, sweet corn and waxy corn. Optimum activities using guaiacol as the hydrogen donor were obtained for s ... mehr
Die Max-Planck-Gesellschaft und Springer haben sich einvernehmlich darauf verständigt, den Wissenschaftlern aller Max-Planck-Institute und Forschungsgruppen den Zugriff auf sämtliche Inhalte der Onlineplattform SpringerLink zu ermöglichen.
Die Vereinbarung umfasst auch Open ChoiceTM, das O ... mehr
Mit mehr als 18.000 elektronischen Laborprotokollen in den Bereichen Life Sciences und Biomedizin hat der Wissenschaftsverlag Springer sein Online-Angebot für Forschungslabors erheblich ausgebaut. Unter dem Namen Springer Protocols finden Wissenschaftler aus Forschungsinstitutionen und Unte ... mehr
Die Max-Planck-Gesellschaft hat nach schwierigen Verhandlungen ihren langjährigen Lizenzvertrag mit dem Springer Verlag zum 31. Dezember 2007 gekündigt. Die Verhandlungen sind gescheitert, weil laut MPG keine Einigung über ein adäquates Verhältnis von Preis und nachhaltigen Leistungen erzie ... mehr