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Methods for single-cell analysis are critical to revealing cell-to-cell variability in biological systems, especially in cases where relevant minority cell populations can be obscured by population-averaged measurements. However, to date single cell studies have been limited by the cost and throughput required to examine large numbers of cells and the difficulties associated with analyzing small amounts of starting material. Microfluidic approaches are well suited to resolving these issues by providing increased senstitivity, economy of scale, and automation. After many years of development microfluidic systems are now finding traction in a variety of single-cell analytics including gene expression measurements, protein analysis, signaling response, and growth dynamics. With newly developed tools now being applied in fields ranging from human haplotyping and drug discovery to stem cell and cancer research, the long-heralded promise of microfluidic single cell analysis is now finally being realized.
Autoren:
Véronique Lecault, Adam K White, Anupam Singhal, Carl L Hansen
Publication date: Available online 2 May 2013Source:Computational Biology and Chemistry Author(s): Jimmy Franco , Margaret A.L. Blackie , David Toth , Peter J. Smith , Joseph Capuano , Kurt Fastnacht , Charlotte Berkes Malaria continues to affect millions of people annually. With ... mehr
Publication date: Available online 29 April 2013Source:Current Opinion in Chemical Biology Author(s): Daniel J Scott , Lutz Kummer , Dirk Tremmel , Andreas Plückthun Integral membrane proteins (IMPs) are crucial components of all cells but are difficult to study in vitro because the ... mehr
Publication date: Available online 3 May 2013Source:Current Opinion in Chemical Biology Author(s): Jayanta Chatterjee , Maja Köhn Protein phosphatase-1 (PP1) has long been neglected as a potential drug target owing to its misinterpreted unselective nature. However, growing evidence de ... mehr
Der Spektrum Akademischer Verlag, Teil des Elsevier-Verlags, hat sich entschlossen, sich auf sein Kerngeschäft zu konzentrieren und die Seminartätigkeit zum 01.01.2007 aufzugeben.
In der PromoCell Academy sieht der Verlag einen Partner und Nachfolger mit Kernkompetenz "Schulungen im naturw ... mehr
Elsevier, Herausgeber wissenschaftlicher , technischer und medizinischer Information, gab bekannt, dass das Unternehmen die erste Sitzung des Biowissenschaftsbeirates am Freitag, den 24. Juni im Hauptsitz des Unternehmens in Amsterdam einberufen hat. Der Beirat besteht aus einer Gruppe inte ... mehr