Bionity.COM Articles

High sensitive detection of follistatin by Imperacer�: an initial study on the way to analytical gene-doping tests

For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological ...

Molecular Probes� NanoOrange� Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader

The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three ...

Rapid High-Throughput Methylation Analysis Using the LightCycler� 480 System

The LightCycler� 480 Instrument is a versatile platform for molecular studies. We assessed the performance of the instrument combined with the LightCycler� 480 High Resolution Melting Master mix for DNA methylation analysis using the ...

Quantitative Chromatin Immunoprecipitation Using the LightCycler� 480 System

Introduction

Antigen processing and presentation play a critical role in immune surveillance. Peptides derived from self, viral, or tumor-associated antigens are generated in the cytoplasm by the proteasome. ...

Methylation of the Arginine 2 in Histone H3 Controls Deposition of Lysine 4 Tri-Methylation

In a recent study, Kirmizis et al. demonstrated that H3R2 is methylated not only in mammals but also in Saccharomyces cerevisiae. By using a specific H3R2me2a antibody in a ChIP-on-Chip analysis the authors determined the distribution of the ...

Evolutionary Link Between Maternal Behavior and Eusociality Is Supported by Wasp Gene Expression

A defining feature of eusociality is the presence of workers that abstain from reproduction and care for their siblings (i.e., workers forage for food and feed the brood). It has been proposed that this behavior evolved from maternal care behavior ...

Nature's Ingenuity Shows the Way

Up to 80 percent of our experience of our visual environment takes place via our visual perception. Without spatial vision, we would hardly be able to stay oriented. In recent decades, the neurosciences have gained many insights into the complex ...

Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers

The Dual-Luciferase� Reporter Assay or DLR is widely used to study gene transcription and regulation. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla. The Firefly reaction is initiated, followed by its ...

Bradford Assay Performed on BMG LABTECH�s FLUOstar Omega with new Evaluation Software

Determining the protein concentration of samples is a necessary and often used method in biochemistry. Different colorimetric protein assays have been developed. The most commonly used methods are the Bradford assay, the Lowry assay and the BCA ...

A High-Throughput Experimental Tool for Studying Gene Pathways

The post-genome era has lead to great advances in high-throughput genomics studies. Genomic approaches such as microarray expression analyses and Chromatin Immuno-Precipitation on Chip (ChIP-chip) transcription factor binding assays have yielded new ...

Advances in Transfection Technologies: Using FuGENE� HD Transfection Reagent with PCR Constructs

With recent advances in molecular biology, it is now possible to construct genomic sequences and then evaluate the protein produced by those sequences. Ever-increasing portions of the genome are being investigated for their functions or for the ...

FuGENE� HD Transfection Reagent Provides High Transfection Efficiency in the Murine NB2a Cell Line

Cell transfection is a method of gene delivery that introduces foreign DNA into a cell. It is one of the most important approaches in modern genetic and proteomic research. With intriguing properties, transfection of primary cells is required for ...

Transfecting Mouse Fibroblast NIH 3T3 Cells with Lipid Reagents - A Comparative Study

Prior to the advent of transfection lipids, mouse fibroblast NIH 3T3 cells were essentially untransfectable and other methods such as retroviral gene delivery had to be employed to circumvent this problem. Although retroviruses can be used for ...

New: Multiplex Assays with Universal ProbeLibrary and Assay Design for Many More Organisms

Since the availability of sound sequence data, organisms such as maize, rice, yeast, and zebrafish are gaining more and more importance in academic and industrial research, and the need for specific and quantitative real-time PCR assays for these ...

Confocal Detection of NBT/BCIP In Situ Hybridization

Confocal laser scanning reflection microscopy (CLSM) in combination with whole-mount in situ hybridization (WMISH) is a novel method to visualize gene expression patterns in three dimensions in whole embryos. The method uses conventional NBT/BCIP ...

Transfering PCRs to HRM-assays on the LightCycler� 480 System - Examples for BRCA1

High-resolution melting curve analysis (hrMCA) is an attractive technique to scan for unknown mutations in genes. To evaluate how easy or difficult it is to design hrMCA assays using the LightCycler� 480 Instrument, we selected 3 different fragments ...

New Application for the LightCycler� 480 System: qPCR-based microRNA-Profiling

Recent studies indicate that microRNAs (miRNAs) are mechanistically involved in the development of various human malignancies and therefore represent a promising new class of biomarkers. Quantitative PCR-based technologies are emerging as the gold ...

H5N1: Whole RNA Virus Sequencing Using the Genome Sequencer FLX System

The global spread of the deadly H5N1 avian influenza virus among bird populations and its transmission to humans in endemic areas has emphasized the importance of fast and accurate detection methods for influenza virus strain and variant ...

Effects of Passage Number on Cell Line Transfection

An important factor in the success of any transfection experiment is the quality of the cells. As a key experimental component, the cell line can be the factor with the highest variability, affecting the reliability and reproducibility of results.

Pathogen detection with VYOO(TM)

Sepsis is the most common cause of death in hospitalized patients worldwide. Inadequate antibiotic therapy represents an independent determinant of patients' mortality. Promptness of pathogen detection and the appliance of appropriate antibiotics ...