Bionity.COM Articles

Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlokTMand FLUOstar OPTIMA

Tue, 23 Jun 2009 22:00:00 GMT

The FLUOstar OPTIMA is used for reading the BD Biosciences FluoroBlok(TM) tumour cell invasion system. In this application note we show that the results are comparable to the traditional invasion assays. The introduced method is fast, saving time ...

Fast and Sensitive Detection of Viral Targets in Quantitative RT-PCR Using the Universal One-Step qRT-PCR Master (ROX)

Thu, 11 Jun 2009 22:00:00 GMT

For qRT-PCR assays it is a common procedure to perform two individual work steps. First, RNA is transcribed into cDNA, and then the target to be quantified is amplified separately. This two-step approach is believed to result in highest sensitivity ...

qPCR Identification of Genes Involved in Apoptosis and Cell Cycle Regulation

Tue, 09 Jun 2009 22:00:00 GMT

Systematic high-throughput functional genomic screens with the targeted silencing of genes by the introduction of small interfering RNA (siRNA) have emerged as an extremely powerful tool for analysis of biological functions. Such screens commonly ...

The New MagNA Pure LC Total Nucleic Acid Isolation Kit

Mon, 08 Jun 2009 22:00:00 GMT

Many kits and protocols for nucleic acid isolation - manual and automated - are available today. However, very few of them guarantee a fully automated sample preparation, combined with maximum recovery and sensitivity. Therefore we have developed ...

Dynamics of Kinases Involved in Cell Growth Using the xCELLigence System

Sun, 07 Jun 2009 22:00:00 GMT

Studies investigating endogenous cellular regulatory mechanisms have substantially pushed the application of high-throughput screens. RNA interference has developed into an extremely powerful tool for mediating the loss-of-function of specific mRNA ...

Isolation of Keratinocytes and Fibroblasts from Human Foreskin by One-step Enzyme Incubation Using Liberase Research Grade Products

Thu, 04 Jun 2009 22:00:00 GMT

For the in vitro testing of pharmaceutical, cosmetic and chemical products conventional cell cultures are increasingly being replaced by three-dimensional (3D) cell culture models with organ-specific characteristics. Using tissue engineering ...

Outstanding Microarray Experimental Results Using FuGENE� HD Transfection Reagent

Tue, 02 Jun 2009 22:00:00 GMT

Transfection is a method for delivering nucleic acids, either DNA or RNA, into animal cells in vitro. In applications such as protein production, the main purpose is to produce protein; consequently any effects on endogenous gene expression are ...

How much LIMS can a researcher put up with?

Tue, 19 May 2009 22:00:00 GMT

Researchers don't want to work with a LIMS and analysts don't like electronic laboratory notebooks (ELN). These types of software are not ideally suited to mapping these two laboratory processes. In companies with R&D and analysis departments, there ...

Trichostatin A Treatment of Cells Transfected with FuGENE� HD Transfection Reagent

Wed, 13 May 2009 22:00:00 GMT

Treatment of eukaryotic cells with trichostatin A (TSA) - a broad-spectrum inhibitor of histone deacetylation - is frequently used in studies of epigenetic regulation of gene expression. To be able to investigate the effects of TSA on promoter ...

Characterizing Environments: Recent Discoveries Enabled by Metagenomic Analysis with the Genome Sequencer FLX System

Thu, 07 May 2009 22:00:00 GMT

Metagenomics is the study of genomic content in a complex mixture of microorganisms. The two primary goals of this approach are to characterize the organisms present in a sample and identify what roles each organism has within a specific ...

Homogeneous IgG AlphaLISA� assay performed on BMG LABTECH's PHERAstar Plus

Sun, 26 Apr 2009 22:00:00 GMT

Immunoglobulins or antibodies are proteins that are involved in the immune response. That makes them interesting drug targets. This application note shows the detection of the immunoglobulin IgG using an AlphaLISA� assay performed on BMG LABTECH's ...

Cytokine Quantification in Drug Development

Wed, 22 Apr 2009 22:00:00 GMT

Immuno-PCR (IPCR) and ElectroChemiLuminescence (ECL) are technologies for improved performance of immunoassays. The specific advantages of the different technologies, using enzymatic or electrical signal amplification, allow for tailored assay ...

New Standard in Electrophysiology and Deep Tissue Imaging

Tue, 07 Apr 2009 22:00:00 GMT

The function of nerve and muscle cells relies on ionic currents flowing through ion channels. These ion channels play a major role in cell physiology. One way to investigate ion channels is to use patch clamping. This method allows investigation of ...

Outsourcing R&D Innovation takes on New Meaning

Sun, 05 Apr 2009 22:00:00 GMT

Innovative drugs are the lifeblood of pharmaceutical companies, but the cost of developing them is rising to unsustainable levels. Pharma executives are already saying that as the economy falters, they foresee bleak prospects and mergers unless ...

A Rapid, Sample-Safe, Evaporative Technique for Preparation of DNA and Oligonucleotide Samples

Sun, 01 Mar 2009 23:00:00 GMT

When working with DNA and oligo samples great care needs to be taken at every stage to ensure that degradation does not occur, and the highly potent samples are not contaminated. Use of state of the art concentration systems can significantly speed ...

Evidence for a Cysteine Proteinase Involved in BSE Infection and/or Progression

Sun, 14 Sep 2008 22:00:00 GMT

According to the "protein-only" hypothesis, the prion protein seemed to be - for more than a decade - the infectious agent of transmissible spongiform encephalopathies (TSEs) in different species. TSEs are neurodegenerative diseases reported e.g., ...

miRNA Extraction and Profiling on FFPE Samples

Sun, 07 Sep 2008 22:00:00 GMT

RNA silencing is a mechanism of gene regulation whereby small double-stranded RNAs (dsRNAs) of 21-18 nucleotides in length cause mRNA degradation, mRNA translation, or chromatin modification. In the past years, more than 500 novel genes have been ...

The New MagNA Pure LC 2.0 System: New Design and Improved Performance Combined with a Proven Nucleic Acid Isolation Technology

Sun, 31 Aug 2008 22:00:00 GMT

The fast, flexible, and reliable isolation of pure nucleic acids from various sample materials is crucial for sensitive molecular analysis by PCR, RT-PCR, sequencing, arrays etc. In addition, automation of nucleic acid isolation is becoming ...

Predictor(TM) hERG Fluorescence Polarization Assay Kit performed on the PHERAstar/PHERAstar Plus

Wed, 27 Aug 2008 22:00:00 GMT

The potential for cardiotoxic side-effects continues to challenge the development of small molecule based therapies. These intolerable side-effects are often precipitated by drug-induced long QT syndrome (LQT), which is often linked to blocking the ...

Optimized Production of Lentivirus Using FuGENE� HD Transfection Reagent

Tue, 26 Aug 2008 22:00:00 GMT

Lentiviral vectors are ideally suited vehicles for a broad range of research applications. Depending on pseudotype, lentiviruses infect a wide range of cell types, non-discriminately transducing both dividing and non-dividing cells. As opposed to ...