The latest articles with know-how for the biotech, pharma and life science industries http://www.bionity.com/articles/e/ en-us http://www.bionity.com/articles/e/102379/?WT.mc_id=ca0101 The FLUOstar OPTIMA is used for reading the BD Biosciences FluoroBlok(TM) tumour cell invasion system. In this application note we show that the results are comparable to the traditional invasion ... http://www.bionity.com/articles/e/101946/?WT.mc_id=ca0101 For qRT-PCR assays it is a common procedure to perform two individual work steps. First, RNA is transcribed into cDNA, and then the target to be quantified is amplified separately. This two-step ... http://www.bionity.com/articles/e/101945/?WT.mc_id=ca0101 Systematic high-throughput functional genomic screens with the targeted silencing of genes by the introduction of small interfering RNA (siRNA) have emerged as an extremely powerful tool for analysis ... http://www.bionity.com/articles/e/101769/?WT.mc_id=ca0101 Many kits and protocols for nucleic acid isolation - manual and automated - are available today. However, very few of them guarantee a fully automated sample preparation, combined with maximum ... http://www.bionity.com/articles/e/101707/?WT.mc_id=ca0101 Studies investigating endogenous cellular regulatory mechanisms have substantially pushed the application of high-throughput screens. RNA interference has developed into an extremely powerful tool ... http://www.bionity.com/articles/e/101706/?WT.mc_id=ca0101 For the in vitro testing of pharmaceutical, cosmetic and chemical products conventional cell cultures are increasingly being replaced by three-dimensional (3D) cell culture models with organ-specific ... http://www.bionity.com/articles/e/101605/?WT.mc_id=ca0101 Transfection is a method for delivering nucleic acids, either DNA or RNA, into animal cells in vitro. In applications such as protein production, the main purpose is to produce protein; consequently ... http://www.bionity.com/articles/e/100634/?WT.mc_id=ca0101 Researchers don't want to work with a LIMS and analysts don't like electronic laboratory notebooks (ELN). These types of software are not ideally suited to mapping these two laboratory processes. In ... http://www.bionity.com/articles/e/100686/?WT.mc_id=ca0101 Treatment of eukaryotic cells with trichostatin A (TSA) - a broad-spectrum inhibitor of histone deacetylation - is frequently used in studies of epigenetic regulation of gene expression. To be able ... http://www.bionity.com/articles/e/100685/?WT.mc_id=ca0101 Metagenomics is the study of genomic content in a complex mixture of microorganisms. The two primary goals of this approach are to characterize the organisms present in a sample and identify what ... http://www.bionity.com/articles/e/100245/?WT.mc_id=ca0101 Immunoglobulins or antibodies are proteins that are involved in the immune response. That makes them interesting drug targets. This application note shows the detection of the immunoglobulin IgG ... http://www.bionity.com/articles/e/100005/?WT.mc_id=ca0101 Immuno-PCR (IPCR) and ElectroChemiLuminescence (ECL) are technologies for improved performance of immunoassays. The specific advantages of the different technologies, using enzymatic or electrical ... http://www.bionity.com/articles/e/99538/?WT.mc_id=ca0101 The function of nerve and muscle cells relies on ionic currents flowing through ion channels. These ion channels play a major role in cell physiology. One way to investigate ion channels is to use ... http://www.bionity.com/articles/e/99167/?WT.mc_id=ca0101 Innovative drugs are the lifeblood of pharmaceutical companies, but the cost of developing them is rising to unsustainable levels. Pharma executives are already saying that as the economy falters, ... http://www.bionity.com/articles/e/97625/?WT.mc_id=ca0101 When working with DNA and oligo samples great care needs to be taken at every stage to ensure that degradation does not occur, and the highly potent samples are not contaminated. Use of state of the ... http://www.bionity.com/articles/e/86341/?WT.mc_id=ca0101 According to the "protein-only" hypothesis, the prion protein seemed to be - for more than a decade - the infectious agent of transmissible spongiform encephalopathies (TSEs) in different species. ... http://www.bionity.com/articles/e/86294/?WT.mc_id=ca0101 RNA silencing is a mechanism of gene regulation whereby small double-stranded RNAs (dsRNAs) of 21-18 nucleotides in length cause mRNA degradation, mRNA translation, or chromatin modification. In the ... http://www.bionity.com/articles/e/86245/?WT.mc_id=ca0101 The fast, flexible, and reliable isolation of pure nucleic acids from various sample materials is crucial for sensitive molecular analysis by PCR, RT-PCR, sequencing, arrays etc. In addition, ... http://www.bionity.com/articles/e/86154/?WT.mc_id=ca0101 The potential for cardiotoxic side-effects continues to challenge the development of small molecule based therapies. These intolerable side-effects are often precipitated by drug-induced long QT ... http://www.bionity.com/articles/e/86225/?WT.mc_id=ca0101 Lentiviral vectors are ideally suited vehicles for a broad range of research applications. Depending on pseudotype, lentiviruses infect a wide range of cell types, non-discriminately transducing both ... © 2009, Chemie.DE Information Service GmbH.